I am currently looking into magnesium transporter and TRPM7 currents in Jurkat cells. I been performing whole-cell patch clamp and perforated patch on Jurkat cells with and without mutations in those channels I mention. Along with changing the external solution as well.
Haha frankly I just started this project about 2 months ago so I am still unsure of the details ¯\_(ツ_/¯
BTW I hate patch clamping. Even though I was able to pick it up "fast".
I see, I always liked immunology aspects. I actually did handle some macrophages for calcium imaging when I first started my lab rotation in my current lab.
Haha true dat. I guess after doing the same thing hours during a day, you should pick up fast on it But for real patching a tiny ass cell is a pain!
Well regardless, I wish you the best of luck and let me know when you get in
BTW we should keep in contact as fellow neuroscience students :3
that makes it at least 4 people in Neuroscience here, eh
I want to get into more flow cytometry (only use one laser) and wasnt really using it for the traditional sorting with antibodies, used it to quantify protein in cells.
Western Blotting and is my fav technique. Followed by ELISAs.
Do you have to manually suck to break the membrane?
I had to do that for a lab rotation and could never get the hang of it
Always so weird to me that you literally sucked open a whole in a cell haha
I'll definitely stay in touch with you guys! Always good to hear from fellow neuro people.
Yeah, we have two different flow cytometers in our facility, one for regular flow and one for cell sorting. Each has three lasers for up to 8-9 colors. Things can get really complicated, real fast, especially when people don't bring in proper controls and then it's impossible for me to help them out with compensation of fluorescent spillover.
I do enjoy doing some immunoassays. We got a multiplex immunoassay instrument a little while back that's been fun to mess with.
Was doing a western blot today and was wondering why my protein just wasnt transfering even after four hours...didnt know the new Immunoblot pvdf membrane I ordered had to be activated with methanol. Why cant bio rad include instructions with orders
Publishing sucks. Anyone else agrees!?
Publishing sucks. Anyone else agrees!?
Curious if anyone has any thoughts on the worthwhileness of a PhD in IT? Recently completed my Masters in IT, but wasn't sure how much return on investment I would see if I attempted a PhD. Feel like a lot of advanced IT jobs look more favorably on experience over multiple degrees.
Dont... all... pvdf membranes have to be activated with methanol first? But yeah that's an easy thing to forget about, at least you only wasted a day on it!
Curious if anyone has any thoughts on the worthwhileness of a PhD in IT? Recently completed my Masters in IT, but wasn't sure how much return on investment I would see if I attempted a PhD. Feel like a lot of advanced IT jobs look more favorably on experience over multiple degrees.
Edit: Part of me thinks it would be enjoyable - in masochistic sort of way - to get the PhD. The other part of me thinks I should just focus on programming/application dev in my own time, eventually making a career shift (in healthcare IT now).
That or be someone who is independently wealthy with nothing else better to do in their lives.
Gaf, sorry if this isn't graduate enough for this thread but my masters degree is not going well. At all. I work so hard all the time to just scrape through and I'm so stressed and I'm just not good enough and I barely see my friends or my family any more and I never have time to exercise and today my sensei chewed my out in front of a bunch of other people because my martial arts skills have atrophied and I hate everything and I just don't see the point in anything anymore and I don't wanna carry on and keep trying so hard at every area of my life only to fail at all of them.
I just feel so useless and pathetic.
So, people with experience here's my question: does it get any better? or is it all downhill from here?
Gaf, sorry if this isn't graduate enough for this thread but my masters degree is not going well. At all. I work so hard all the time to just scrape through and I'm so stressed and I'm just not good enough and I barely see my friends or my family any more and I never have time to exercise and today my sensei chewed my out in front of a bunch of other people because my martial arts skills have atrophied and I hate everything and I just don't see the point in anything anymore and I don't wanna carry on and keep trying so hard at every area of my life only to fail at all of them.
I just feel so useless and pathetic.
So, people with experience here's my question: does it get any better? or is it all downhill from here?
I'm in school psychology now! Realized it's not for me.. so I just submitted all my applications for social psychology doctorate programs on dec 1st.Got my school psychology applications submitted along with all my supplemental forms and LoR. Deadline for my main universities are the 15th, and New Years. Now the waiting game begins.
Publishing sucks. Anyone else agrees!?
Feels like 500 years, but it's only been a couple of months.How long have you been working on your masters?
Ah I'm sorry man! Please don't let me put you off from following your dreams, I'm just personally overwhelmed at the moment.I'm currently an undergrad with my masters next year, and yeah you're making everything sound terrifying!
Maybe the key is time management? I've had a load of assignments the last month and haven't done any exercise or even proper shopping. Meanwhile if I spent maybe 20 minutes at the start of the week nailing down when and where things would get done I would've probably found the time to do that shit.
But you're gonna be way more overworked than me. Projects are hard and there are low times, but the next breakthrough will be worth it. Hope this helps man, I'm pulling for you!
I'm in school psychology now! Realized it's not for me.. so I just submitted all my applications for social psychology doctorate programs on dec 1st.
Edit: with that being said, there's such a shortage of school psychologists across the nation, so you should have no problem getting accepted. Best of luck!
Or maybe it's just something you enjoy doing.
I hate being career minded, I don't think I could ever do it.
i hear the first semester is usually the worst for a lot of people. hang in there man, you'll get the hang of it.Feels like 500 years, but it's only been a couple of months.
Ah I'm sorry man! Please don't let me put you off from following your dreams, I'm just personally overwhelmed at the moment.
Time management would absolutely help, yeah. But I always get easily distracted by shiny things and really don't have the discipline to work at this stuff.
Cheers for the support! I'm just dealing with this at the same time my personal life is becoming a train wreck so it's a bit overwhelming.
i hear the first semester is usually the worst for a lot of people. hang in there man, you'll get the hang of it.
starting my master's next spring, super excited since I'll be moving for it and I'll have a gta position
I definitely feel the heat nearing the end of my first semester. In some ways I feel like I have hit a wall where Physics has become too complex for me, but I could just be being hard on myself. Still I look around at the other students and they seem to be thriving while I feel like I am crashing and failing. For the first time ever I didn't complete an assignment because I just didn't have time and found it too difficult and it felt awful.
Ah I'm sorry man! Please don't let me put you off from following your dreams, I'm just personally overwhelmed at the moment.
Feels like 500 years, but it's only been a couple of months.
My program is a joint M.Ed/EdS program, and I'll have my EdS after finishing my internship in June. Although I likely won't stay in this field (you never know, though..) I'm extremely thankful for the opportunities it has awarded me working with such diverse populations.Your edit is partly why I decided to pursue the field. All of my applications are for NASP-accredited schools, so I have the opportunity to work in a blue-heavy state.
Florida is on point for their programs. One of my choices includes a master's degree in HR once one finishes their EDS program.
RIPSemesters with taking classes + teaching three classes + lab research + writing a research plan and doing a committee meeting were the worst for free time. Not enough hours in a day.
So far having a Master's Degree has done zero favors for me in the job seeking department... I did frame it and put it on my wall though.
Do jobs in your field often ask for a master's?So far having a Master's Degree has done zero favors for me in the job seeking department... I did frame it and put it on my wall though.
M.S. in Education.What kind of degree?
It's actually a requirement in the teaching profession after a certain point. You can start out without one, but they make you go get one later (I think you need one by four years after getting certified or something like that). I'm just starting out though.Do jobs in your field often ask for a master's?
Between my undergrad and graduate school, I've pulled through 17 semesters total. Seventeen.
Yikes.